Describe the process of using reassorted genomes as a method to attenuate live viral vaccines. List one vaccine that has been developed using this strategy

Describe the process of using reassorted genomes as a method to attenuate live viral vaccines. List one vaccine that has been developed using this strategy

Describe methods to develop a temperature sensitive viral mutant vaccine strain. List one vaccine that has been developed using this strategy

List three reasons limiting the use of classical methods to attenuate bacterial vaccine strains

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