SIMPLE STAIN AND NEGATIVE STAIN
1. Explain why a simple stain colorizes the cells while a negative stain does not.
2. Sometimes you can stain with both a simple stain and a negative stain at the same time. If stained a smear of Bacillus subtilis with both safranin (pink) AND nigrosin (dark purple/black negative stain) what would I expect to see on the slide? Draw me a picture. Be sure to address the shape of the bacteria in your drawing.
3. How about a smear of Streptococcus pyogenes with crystal violet (purple)? Draw a picture. Be sure to address the shape and arrangement of the bacteria in your drawing .
4. How about a smear of Staphylococcus aureus with Congo red? Draw a picture. Be sure to address the shape and arrangement of the bacteria in your drawing
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