Briefly state what the poster is about in approximately 250 words. State what the subject is, why it’s important and give some background information. Use references to support your document.

Metabolism and Tumour Biology

Description

This coursework involves producing an A1 format, portrait orientation poster at the University symposium on Metabolism and Tumour Biology on a case study

You must:

– Analyse, describe and interpret the findings in the case study

– The introduction should briefly state what the poster is about.

– The numerical data in the tables should be analysed and presented graphically along with any statistical analysis of the data you perform

– The photomicrographs should be labelled

– Each figure will have a succinct legend to describe the key messages of the figure

– Conclude with a section on the observations made, the likely prognosis and treatments possible for the individual

You will be assessed on:

– The poster’s appearance (including text, layout) – The poster needs to be eye-catching and clear

– Your analysis (calculations, graphs and stats)

– Your data interpretation

Introduction/Background:

The introduction should briefly state what the poster is about in approximately 250 words. State what the subject is, why it’s important and give some background information. Use references to support your document.

– Introduce the discussion points

– Frame objectives

– Make your thesis statement

Figure 1: Mutant isocitrate dehydrogenase (IDH1) sequence

(DNA sequences obtained after PCR analysis of DNA extracted from patient’s glioma biopsy)

– You must use BLAST to identify the mutation of the protein

Figure 2. IDH1 enzymatic activity detected in untransfected (Parental) cells, cells expressing IDH1 wild-type (WT) or IDH1 Mutant

(The table – Numerical data)

How will you analyse and display this data?

How can you tell if there is a difference between the different samples? (Statistical analysis)

What does this tell you about the activity of the enzyme?

The figure will be the analysis of the data in table 1: the enzymatic activity of wild-type and mutant DH1 proteins.

Write a figure legend and describe and interpret the results.

– Include the graph not the table

Figure 3: Anti-IDH1 immunohistochemistry

(The micrograph)

What does this show?

– Label each figure and write a legend of the key message that is shown in the figure

Figure 4: Levels of 2-HG in normal brain tissue and IDH1-mutated patient glioma tissue

(The table)

– How will you analyse and display this data?

– How can you tell if there is a difference between the different samples

– What does this tell you?

This will be graphical representation of the data in figure 4: The levels of 2-HG. Give the figure a title and a legend. What do they mean?

– What is 2-HG

– How is it related to the patient

– Why is there experimental data on 2-HG

Figure 5: Measurement of HIF1a activity in normal brain tissue and IDH1-mutated patient glioma tissue.

– How will you analyse and display this data?

– How can you tell if there is a difference between the different samples

– What does this tell you?

This will be graphical representation of the data in figure 5

Give the figure a title and a legend.

– What is HIF1a activity

– Why are we measuring HIF1a activity and conducting this experiment

Figure 6: Expression levels of cyclin D1, vascular endothelial growth factor (VEGF) and platelet-derived growth factor A (PDGFA) in IDH1-mutated tumor lysates obtained from glioma patient and normal brain

(Western blot)

– What does this show?

– How is this related to the patient and the other data

– What does this mean for the patient?

Conclusions:

– Make your conclusions in a number of bullet points

– Interpret the results in the light of other published results and provide possble explanations for the data.

– What is the likely prognosis and treatments possible for the patient?

– Use the space effectively without waffle, i.e be concise but evaluate and discus all the data critically

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