Calculate the change in absorbance between Time 0 and Time 50 from the Benedicts test and place the results in Table 4.

Biology Report
The data you will need to answer the following questions are listed in Table 1.
Table 1: Color, estimated pH and absorption every 10 min from the bicarbonate solution.
Time (min)
W Syringe
G Syringe
Color
Estimated pH
Absorption Å
Color
Estimated pH
Absorption Å
0

Red/Purple
8.8
0.779
Red/Purple
8.8
0.761
10

Red/Purple
8.8
0.734
Red
8.6
0.624
20

Red/Purple
8.8
0.751
Red
8.6
0.603
30

Red/Purple
8.8
0.753
Dark 0range
8.4
0.583
40

Red/Purple
8.8
0.753
Dark orange
8.4
0.504
50

Red/Purple
8.8
0.751
Orange
8.2
0.433

Time (min)
Mg Syringe
Color
Estimated pH
Absorption Å

0

Red/Purple
8.8
0.729
10

Red
8.6
0.607
20

Dark 0range
8.4
0.529
30

Orange
8.2
0.407
40

Orange/Yellow
8.2
0.334
50

Orange/Yellow
7.8
0.308

2) (2 pnts) Calculate the change in absorption using the formula ABS0 – ABST (Absorption at time 0 min – the Absorption at time T min) and place the values in Table 2.

Table 2: Change in absorption over time for the bicarbonate solutions.
Time (Min)
W Syringe
G Syringe
Mg Syringe
0

0
0
0
10

20

30

40

50

3) (4pnts) Graph (use scatter plot) the change in absorption over time for all 3 treatments (W, G, Mg) and paste the graph below. Be sure to label the axes.

4) (3pnts) Which reaction produced CO2 the fastest? Use the rate of color change and the change in absorption to answer the question.

Data collected from conducting the Benedicts test are listed in Table 3.

Table 3: Results of Benedicts test
Treatment
Time 0 Absorption Å
Time 0 Color
Time 50 Absorption Å
Time 50 Color
W

0.021
Blue
0.022
Blue
G

0.593
Orange/Red
0.379
Yellow/Orange
Mg

0.589
Orange/Red
0.182
Green/yellow

6) (1pnts) Calculate the change in absorbance between Time 0 and Time 50 from the Benedicts test and place the results in Table 4.

Table 4: Change in absorbance in the Benedicts test
Treatment
Time 0 – Time 50 Å

W

G

Mg

7) (4pnts) Which reaction showed the loss of most of the glucose? Justify your answer with the data you collected.

8) (4pnts) Based on the both data sets (bicarbonate and Benedicts) what affect did Mg have on the rate of the reaction? Justify your answer with both data sets. (Note: if you know there was an issue with some of your data you can explain what you expected to happen and why your results do not match the expectation).

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