Compare the molecular weights obtained for your three prominent bands by using Rf values to the MW obtained by MALDI-TOF. How much discrepancy (if any) did you see between the two methods?
Guidelines for the Affinity Purification Lab Report (lab report 4, to be written individually) 1) Estimate the molecular weights (using Rf values) of the three prominent bands you see in your thrombin digest lane. 2) You have been provided with two results of a MALDI-TOF analysis (pp. 102-103 above). a) Calculate the actual molecular weight […]