Watch the JoVe video entitled anion exchange chromatography. Answer the following questions.
a) Why is acid required for the mobile phase in anion exchange? Draw a picture of the
anion exchange column in the presence of a neutral, positively charged, and negatively
charged analyte and explain what happens.
b) Again using a picture, indicate how salt can be used to unbind the analyte.
2. To prepare for lab, go through each step and consider how to connect what you will do and observe with chemistry taking place. The pre-lab will be graded on completion.
The post-lab will be graded on correctness.
For each of the following steps in the procedure, answer the following questions.
Step 3: Draw the column and its contents at the beginning of this step. Draw or indicate any colors observed and what chemical species relate to each color.
Draw the column and its contents at the end of this step. Draw or indicate any colors observed and what chemical species relate to each color.
Step 4: Draw the column and its contents at the beginning of this step. Draw or indicate any colors observed and what chemical species relate to each color.
Draw the column and its contents at the end of this step. Draw or indicate any colors observed and what chemical species relate to each color.
Step 5: Write out the chemical reaction that occurs during the preparation of your unknown for chemical analysis. Below each product and reactant, indicate it color in solution.
Step 6: Write out the chemical reaction that occurs during the preparation of your unknown for chemical analysis. Below each product and reactant, indicate it color in solution.
