How might you break CCS to determine what is necessary for CCS’s function? List the residue (amino acid) and it’s location (or what you would break), the reason you chose the location. Design primers that you would use to for site-directed mutagenesis to mutate that residue.
Molecular Biology lab Testing the phenotypes Your goal is to test your strains that you made in the yeast transformation lab. You will do this by comparing growth of a wild-type strain, a strain missing Sod1 (sod1∆), and a strain missing CCS (ccs∆). (SOD1 = gene; sod1∆ = gene has been knocked out or removed […]