Assuming that each colony arose from one molecule of DNA, what percentage of the DNA molecules were successfully transformed? What might limit the number of colonies obtained from the transformation?Provide an explanation forany changes in the color of the colonies on the plates for the transformations.

Lab Report III (Labs 3-5)Calculate the transformation efficiencies for both ligation reactions:Transformation efficiency = (number of colonies)/(μg DNA)To do this, the amount of DNA that resulted in the number of colonies on each plate needs to be calculated. Remember that not all of the transformation mixture was plated. If no colonies were observed on a […]

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